电针对氯胺酮依赖大鼠杏仁核一氧化氮合酶及生长抑素mRNA表达的影响

EFFECTS OF ELECTROACUPUNCTURE ON NOS AND SOM mRNA EXPRESSION OF AMYGDALOID NUCLEUS IN KETAMINE INHIBIT RATS

目的:探讨电针治疗氯胺酮(KTM)依赖的可能机制。方法:将32只SD大鼠随机分为正常对照组,KTM组,KTM加电针组,分别进行相应给药及处理。采用还原型尼克酰胺嘌呤二核苷酸脱氢酶(NAD-PH-d)法、原位分子杂交法分别显示杏仁核内一氧化氮合酶(NOS)、生长抑素(SOM)mRNA的表达。结果:KTM可抑制杏仁核基底外侧核群(ABL)和皮质内侧核群(AME)内NOS及各亚细胞群内SOM mRNA的表达,电针可调高其表达,有统计学意义(P<0.05或P<0.01)。结论:电针对一氧化氮(NO)、SOM mRNA表达的调节可能是治疗KTM依赖的机制之一。

Objective:To explore the mechanism of electroacupuncture on ketamine inhibit rats.Methods:32 SD rats were divided into 3 groups,normal,ketamine and electroacupuncture groups at random. Each group except normal was intraperitoneally injected ketamine once a day for 7 days,electroacupuncture group acupuncturing in zusanli point and conducted for 30 minutes at 2 Hz frequency once a day for 7 days. Nicotinamide adenosine dinucleotide phosphate diaphorase(NADPH-d) and situ-hybridization methods were used to dete...