摘要
目的:观察丹参注射液(SMI)对大鼠心肌缺血-再灌注损伤诱导的细胞凋亡的影响并探讨其机制。方法:采用Langendorff离体灌流装置复制大鼠心肌缺血-再灌注损伤模型。实验随机分为正常对照组(Control),缺血-再灌注损伤组(I/R),丹参注射液保护组(SMI)。生化法检测冠脉流出液乳酸脱氢酶(LDH)的活性和组织匀浆超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量;免疫组化法检测Bcl-2、Bax和caspase-3的表达变化;采用缺口末端标记法(TUNEL)检测心肌细胞凋亡情况;电镜观察心肌组织超微结构。结果:与正常对照组比较,缺血-再灌注损伤组冠脉流出液中LDH漏出增加;组织匀浆中SOD的活性降低、MDA的含量增加;Bcl-2表达减少,Bax和caspase-3表达均增加;细胞凋亡加重;心肌超微结构损伤加重。丹参注射液可以减轻心肌缺血-再灌注损伤,表现为冠脉流出液中LDH漏出减少;组织匀浆中SOD的活性升高、MDA的含量降低;Bcl-2表达增加;Bax和caspase-3表达均减少;细胞凋亡减轻;心肌超微结构损伤减轻。结论:丹参注射液通过下调Bax和caspase-3基因表达,上调Bcl-2基因表达抑制心肌缺血-再灌注损伤诱导的细胞凋亡。
Objective:To study effect and mechanism of(Salviae Miltiorrhizae injections SMI)on cardiomyocytes apoptosis induced by ischemia-reperfusion in rat.Methods:The rat cardiac I/R injury model in vitro was rebuilt on Langendorf device.Experiments were randomly divided into 3 groups:control group,ischemia-reperfusion(I/R)group,Salviae Miltiorrhizae injections(SMI)group.The activity of LDH,SOD and the contents of MDA were measured,the expression of Bcl-2,Bax and caspase-3 was detected by using immuno-histochemical...
出处
《中医药学报》
CAS
2009年第4期14-17,101,共5页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省科技厅国际合作课题资助(No.WC02303)
关键词
丹参注射液
缺血-再灌注
细胞凋亡
大鼠
Salviae miltiorrhizae injections
Ischemia-reperfusion
Apoptosis
Rat