双氢杨梅素对大鼠肝癌前病变模型抗氧化酶活性的影响

Effects of Dihydromyricetin on the Serum and Liver Antioxidative Activities in Initial Stage of Rat Chemical Hepato-carcinogenesis

目的:探讨双氢杨梅素在大鼠肝脏化学致癌初期对血清及肝脏抗氧化酶活性的影响,从而进一步探索其抗致癌作用的可能机制。方法:按略改良的Soh Farber方法建立大鼠肝脏癌前病变模型,用双氢杨梅素饲养大鼠6周后,取血及肝脏测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽-S-转移酶(GSH-ST)、过氧化氢酶(CAT)、丙二醛(MDA)及γ-谷氨酰转肽酶(γ-GT)。结果:双氢杨梅素在大鼠肝脏化学致癌初期对血清和肝脏中SOD、GSH-Px、GSH-ST、CAT的活性有明显回升作用,降低MDA含量,并能抑制癌前病变标志物γ-GT活性表达。结论:双氢杨梅素具有抗氧化和抑制癌前病变标志物的作用,可能是双氢杨梅素的抗致癌机制之一。

Objective:To study effect from extract of dihydromyricetin on serum and liver antioxidative activities and r-glutamine trans-peptidase(r-GT)activity in initial stage of rat chemical hepato-carcinogenesis and possible mechanism of its anti-carcinogenesis.Methods:Hepatic preneoplastic lesions in rats were induced by modified SOh-farber protocol.The activities of serum superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),gluta-thione-S-transferase(GSH-ST),catalase(CAT),r-glutamine transpeptidase(r-GT)and t...