洛铂体外诱导人结肠癌细胞株LOVO细胞凋亡及其作用机制

Lobaplatin-induced Cell Apoptosis of Human Colon Carcinoma Cell Line LOVO in vitro: Role and its Mechanism

[目的]探讨洛铂体外诱导人结肠癌细胞株LOVO细胞凋亡及其作用机制。[方法]采用四甲基偶氮唑盐(MTT)法测定不同浓度(500μM、1000μM、2000μM)的洛铂作用于LOVO细胞24h的生长抑制率;原位末端标记技术法(TUNEL)检测LOVO细胞凋亡指数,计算凋亡阈值;流式细胞技术检测细胞周期变化;分光光度法检测半胱氨酸天冬氨酸蛋白酶(caspase)-3、8、9的表达。[结果]洛铂体外能抑制人结肠癌细胞株LOVO细胞的增殖,且呈剂量依赖性(P<0.05);洛铂能诱导LOVO细胞发生凋亡,凋亡阈值为1000μM;流式细胞技术分析显示洛铂致LOVO细胞周期明显阻滞在S期;分光光度法提示洛铂作用后LOVO细胞caspase-3、8、9表达明显高于阴性对照组(P<0.05)。[结论]洛铂能诱导体外培养的LOVO细胞凋亡,其作用机制可能与洛铂诱导LOVO细胞S期阻滞及促进caspase-8和caspase-9表达,进而促进cas-pase-3表达有关。

[Purpose] To investigate the cell apoptosis induced by lobaplatin in human colon carcinoma cell line LOVO in vitro and its mechanism. [Methods] The rate of LOVO cell growth inhibition in 24 hours was determined by MTT on different dose of lobplation (500μM, 1000μM, 2000μM). Apoptotic index was detected by TUNEL and the threshold value of lobaplatin was calculated. Cell cycles and apoptosis were examined by flow cytometry (FCM) and activities of caspase-3, 8, 9 by chromometry. [Results] The MTT colormetric a...