重型β地中海贫血造血干细胞移植排斥与受者细胞免疫功能的关系

Relationship between cellular immune function during conditioning and graft rejection in patients with beta-thalassemia major

目的研究重型β地中海贫血患儿造血干细胞移植预处理前后的细胞免疫功能变化,分析其与移植排斥之间的关系。方法测定25例次重型β地中海贫血患儿预处理前后外周血中CD3+、CD3+CD4+、CD3+CD8+、CD3-CD56+细胞比率,以同期进行异基因造血干细胞移植的11例急性白血病患儿作对照,研究移植排斥与细胞免疫功能之间的关系。结果重型β地中海贫血组植入率65.2%,排斥率34.8%,急性白血病组植入率100%;CD3+CD8+T细胞百分比,重型β地中海贫血组在预处理前、后均高于急性白血病组(P均<0.05);CD3-CD56+细胞比率,重型β地中海贫血组预处理后较预处理前升高(P<0.05),而急性白血病组预处理后较预处理前显著降低(P<0.05);预处理前CD3-CD56+NK细胞比率,重型β地中海贫血排斥组高于植入组(P<0.05)。结论CD3-CD56+NK细胞可能与重型β地中海贫血移植排斥有关,可做为预测排斥的指标之一;CD3+CD8+T细胞与β地中海贫血移植排斥的关系有待进一步探索。

Objective To analyze the relationship between cell-mediated immune function during conditioning and graft rejection in patients with beta-thalassemia major.Methods Allogeneic hematopoietic stem cell transplantation was performed in 25 children with beta-thalassemia major and 11 with acute leukemia group.The percentages of T lymphocytes and natural killer(NK) cells in peripheral blood of these patients were detected with dual color immunofluorescence on day-10(before conditioning) and day-5(after conditionin...