转化生长因子β对肝星状细胞c-fos基因表达的调节

Regulation of c-fos gene expression in hepatic stellate cells by transforming growth factor beta

目的了解转化生长因子β(TGF)对肝星状细胞c-fos基因表达的影响。方法在培养的肝星状细胞系中加入不同浓度的TGF(终浓度0.2、1、5ng/ml),于8、24、48、72h四个时间点分别收集细胞,提取肝星状细胞总RNA;用逆转录定量PCR方法测定c-fos基因的表达水平。结果TGF不同浓度组肝星状细胞c-fos基因表达水平在8、24、48、72h四个时间点均显著高于对照组;随剂量加大,c-fos基因表达水平逐渐升高;3组间也有显著差异。结论转化生长因子β对肝星状细胞c-fos基因表达有明显的上调作用。

Objective To investigate the effects of transforming growth factor beta(TGF) on c-fos gene expression in hepatic stellate cells.Methods Hepatic stellate cells(HSC-T6) were cultured in the medium containing different concentrations of TGF(0.2,1,and 5 ng/ml),and cells were collected at different time points of incubation(8,24,48,and 72 h).The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction.Results c-fos gene expression levels ...