含糖腹透液对大鼠腹膜间皮细胞功能的影响

Effects of peritoneal dialysis solution on apoptosis and intracellular free calcium,cell surface ICAM-1 expression of rat peritoneal mesothelial cells

目的探讨含糖腹透液对大鼠腹膜间皮细胞功能的影响。方法体外培养的大鼠腹膜间皮细胞,用流式细胞仪检测细胞凋亡率,同时观察细胞内游离钙的水平和ICAM-1表达水平。结果腹膜透析液刺激1h后,体外培养的大鼠腹膜间皮细胞早期凋亡率增高;随着腹透液中糖浓度越高,刺激时间越长,凋亡率增高越明显,ICAM-1表达显著增加,细胞内游离钙水平下降。结论高糖腹透液可以增加腹膜间皮细胞其凋亡率,降低细胞游离钙水平和增加ICAM-1的表达,这些改变可能是长期腹透病人腹膜纤维化和发生失超滤的原因。

Objective To investigate the effects of peritoneal dialysis solution (PDS) on apoptosis and intracellular free calcium ([Ca2+]i), cell surface ICAM-1 expression of rat peritoneal mesothelial cells (RPMCs). Methods The RPMCs apoptosis rate were determined by flow cytometry. [Ca2+]i in the cells were monitered the fluorescence at 528 nm by confocus laser microscopy. Cell surface ICAM-1 expression were detected by flow cytometry. Results After PDS treatment for 1 h, the RPMCs apoptosis rate were increased. Suc...