摘要
目的观察透射电镜下不同抗结核药物对SD大鼠肾脏超微结构的改变。方法健康成年SD大鼠(清洁级)152只,体重250～300g,雌雄各半,随机分为8组。对照组:0.9%氯化钠溶液;H(INH)组:异烟肼;R(RFP)组:利福平;Z(PZA)组:吡嗪酰胺;HR组:异烟肼+利福平组;HZ组:异烟肼+吡嗪酰胺组;RZ组:利福平+吡嗪酰胺组;HRZ组:异烟肼+利福平+吡嗪酰胺组。大鼠用药的常规剂量:每公斤体重人的常用剂量乘以5.5倍。标本制备:根据各组的不同要求,每天对大鼠进行一次灌胃,10d后断头取血,分离肾脏,戊二醛固定后做电镜检查。结果肾小球细胞部分线粒体溶解、髓样变,粗面内质网脱颗粒,滑面内质网池扩张;肾近曲小管上皮细胞核固缩,细胞器减少。异烟肼、利福平、吡嗪酰胺均可引起大鼠肾脏的损害,多种药物联用比单药应用损害更明显(P<0.01)。结论电镜诊断对抗结核药物所致大鼠肾脏损害有重要价值。
Objective To observe the changes of ultrostructure of kidney caused by antitubercular drugs in SD rats under transmission electron microscope.Methods 152 healthy adult SD rats(clean class),weighted 250～300g,males and females were 50% respectively,who were randomly divided into 8 groups:control group(normal saline);H(INH)group(isonicotinyl hydrazine);R(RFP)group(rifampicin);Z(PZA)group(pyrazinamide);HR group(isonicotinyl hydrazine+rifampicin);RZ group(isonicotinyl+pyrazinamide);HRZ group(isonicotinyl hydrazi...
出处
《河北医药》
CAS
2009年第21期2869-2871,共3页
Hebei Medical Journal
关键词
抗痨药物
SD大鼠
超微结构
透射电镜
肾损害
antituberculosis drugs
sparagne dawley rats
transmission electron microscopy
ultrastructure
renal lesion
