摘要
采用电喷雾质谱(ESIMS)技术,研究了黄酮-7-磷酰化丙氨酸酯(a)、黄酮-7-磷酰化缬氨酸酯(b)、黄酮-7-磷酰化亮氨酸酯(c)、黄酮-7-磷酰化异亮氨酸酯(d)分别与黄酮-7-二异丙基磷酸酯(e)、溶菌酶形成的三体系混合溶液小分子与蛋白大分子的弱相互作用.实验结果表明,在这个三体系的混合溶液中,黄酮-7-磷酰化氨基酸酯a、b、c、d与黄酮-7-二异丙基磷酸酯e相比,与溶菌酶具有更强的弱相互作用能力或亲和力.
In the competition experiment,the non-covalent complexes was studied by electrospray ionization mass spectrometry between the same protein lysozyme and two different kinds of chrysin phosphorylated derivatives,such as Ca and Ce, Cb and Ce,Cc and Ce,Cd and Ce.It was found that,in spite of all of compounds containing phosphoryl group,there were differences among them.The interaction lysozyme and chrysin-7-phosphated amino acid esters(a,b,c,d) was stronger than that of chrysin-7-diisopropyl phosphate(e).
出处
《郑州大学学报（工学版）》
CAS
北大核心
2009年第3期106-109,共4页
Journal of Zhengzhou University（Engineering Science）
基金
国家自然科学基金资助项目(20472076)
河南省杰出青年基金资助项目(0512001400)
关键词
ESI质谱
黄酮-7-磷酰化氨基酸酯
黄酮-7-二异丙基磷酸酯
溶菌酶
竞争作用
electrospray ionization mass spectrometry
chrysin-7-phosphated amino acid esters
chrysin-7-diisopropyl phosphate
lysozyme
competitive interaction
