慢性胃炎患者胃内微生物区系的初步研究

The primary study of the microflora in the chronic gastritis

用从内镜下取得的胃黏膜标本提取总DNA,随后用16S rDNA的方法进行检测,构建系统进化树。我们共从66个有效序列中发现胃内有5个门的细菌种类,其中H.pylori是最为丰富的细菌种类。在胃内苛刻条件下仍存在除了H.pylori之外的复杂的细菌区系,这为我们进一步研究慢性胃炎的治疗开拓了新的思路。

A single gastric mucosal biopsy was obtained from chronic gastritis patients,the extracted genomic DNA was gone through 16S rDNA.We found 10 phylotypes belonged to 5 major phyla.H.pylori was the most abundant phylotype within the libraries.Traditionally,the human stomach had been viewed as an inhospitable environment for microorganisms,with the discovery of H.pylori and other bacterium,the existence of a bacterial community adapting to this human niche becomes more plausible.