柴胡药材中铜 铁 锌含量测定的研究

Determination of Copper Iron and Zinc in Radix Bupleuri

目的:探讨分光光度法测定柴胡中金属元素含量的可行性,并阐述柴胡的药效与金属元素含量可能存在的关系。方法:根据不同的金属离子与特定显色剂显色,产生可见光吸收,采用分光光度法平行测定人发标准品及柴胡中金属元素的含量。结果:柴胡中含丰富的微量金属元素,其中铁含量最高470.05 mg/kg,锌为138.53 mg/kg,铜为63.33 mg/kg。结论:分光光度法操作简便、测量快速、结果准确,易推广使用。柴胡的药效与其中的金属元素种类及含量可能存在密切的联系。

Objective: To discuss feasibility of determining the contents of trace element in Radix bupleuri by spectrophotometry,and to expound potential relationship between the sorts and contents of the elements.Methods: Different metal ions can be colored with specific color reagent,engendering visible light absorption,so spectrophotometry was adopted to determine the contents both of Radix bupleuri and standard substance of human hair.Results: The results showed that Radix bupleuri was rich in trace element,the co...