水稻OsWNK基因超量表达载体的构建及转化

Construction and transformation of super-expressing plasmid of OsWNK gene from rice

选取一个受褐飞虱(Nilaparvata lugensStal)诱导的蛋白激酶基因O sWNK,将该基因的全长cDNA片断通过酶切、连接、转化,连接到pCAMB IA1301质粒表达载体上,成功构建了O sWNK基因的超量表达载体pCAM-B IA1301∶OsWNK.将pCAMB IA1301∶OsWNK电击转入农杆菌细胞中,并将转化好的农杆菌与H1493的愈伤组织共同培养,通过抗生素筛选、PCR鉴定,证明已经获得了转基因植株.

The brown planthopper is one of the most serious pests of rice.Changes such as phenomenon changes,physiology changes,gene expression changes and so on will take place by the piercing-sucking of brown planthopper in rice.In this paper,the full length cDNA of the OsWNK gene induced by brown planthopper was studied,and the plant expression vector pCAMBIA1301∶OsWNK was constructed with the method of digestion,ligation and transformation.Then,the plasmid with the inserted fragment was transformed into the agroba...