摘要
采用氧化铝柱色谱法以95%乙醇-氯仿为洗脱剂分离纯化了肝源性磷脂酰乙醇胺(PE),并采用GF254硅胶板薄层色谱法以氯仿-甲醇-水(65:25:4,体积比)为展开剂检测PE。结果表明,以95%乙醇-氯仿顺序洗脱氧化铝色谱柱中的磷脂时,PE与磷脂酰胆碱(PC)可实现完全分离。采用四甲基偶氮唑蓝比色法(MTT法)测定了在不同时间点25μmol/LPE对人肝癌SMMC-7721细胞增殖的影响,并与子宫颈癌细胞Hela、正常人肝细胞HL7702做比较,发现肝源性PE对肝癌细胞的生长具有明显的抑制作用,且能诱导其发生凋亡。
Phosphatidylethanolamine (PE) in crude phospholipids was purified from swine liver with Al2O3 column with 95% alcohol-chloroform as eluent.PE was determined by thin layer chromatography on GF254 silica gel plate,and eluent solvent was chloroform-methanol-water (65:25:4,v/v).The results showed that PE was completely purified from phosphatidylcholine (PC) by the sequence elution with 95% alcohol and chloroform.The effect of the PE at concentrations of 25μmol /L on the proliferation of human liver cancer SMMC-...
出处
《天然产物研究与开发》
CAS
CSCD
2010年第2期223-227,共5页
Natural Product Research and Development
基金
辽宁省大学生课外学术科技作品竞赛项目
大连医科大学大学生科技活动项目
关键词
磷脂酰乙醇胺
肝癌细胞
增殖抑制
凋亡
猪肝
phosphatidylethanolamine
heptoma cells
proliferation inhibition
apoptosis
swine liver
