摘要
建立了由高分子化合物聚乙二醇(PEG4000)与葡聚糖40000(D40)形成的双水相体系萃取分离杜仲叶中桃叶珊瑚甙的新方法。考察了萃取体系相图,研究了PEG4000/D40质量分数、样品溶液加入量、pH值和温度等因素对双水相成相及桃叶珊瑚甙萃取率的影响。结果表明:PEG4000的质量分数为11%,D40质量分数为8%、样品溶液加入量为8g,温度为60℃,溶液pH为7时,双水相体系对桃叶珊瑚甙有较高的萃取率,重复三次可达到66.32%,而且萃取得到的桃叶珊瑚甙产品的纯度达到48.67%,远远高于粗提物中的8.750%。
A new method to extract and separate aucubin from the leave of Eucommia ulmoides Oliv.in aqueous two-phase system formed by macromolecular compound PEG4000 and dextran 40000 was investigated.The effect of phase map of extraction system,the addition amount of sample solution,pH and temperature were investigated systematically.The results showed that the best ATPS was composed of 11% PEG4000 and 8% D40,and addition of sample was 8 g at 60 ℃,pH 7.The highest extraction rate could reach to 66.32% after three ti...
出处
《天然产物研究与开发》
CAS
CSCD
2010年第2期264-267,共4页
Natural Product Research and Development
基金
湖南省教育厅重点项目(08A056)
张家界市科技局项目(2008)
吉首大学研究生科研创新项目(JGY200907)
关键词
桃叶珊瑚甙
杜仲叶
双水相体系
萃取
分离
aucubin
leave of Eucommia ulmoides Oliv.
aqueous two-phase system
extraction
separation
