盾叶薯蓣内生真菌及其对宿主培养物生长和皂苷元生产的影响

Endophytic Fungi from Dioscorea zingiberensis and Their Effects on the Growth and Diosgenin Production of the Host Plant Cultures

从盾叶薯蓣根状茎中分离并鉴定了9株内生真菌,经悬浮培养14d,分别制备灭活菌丝和菌液浓缩物。其中,内生尖孢镰刀菌Dzf17能有效地提高盾叶薯蓣无菌苗和培养细胞薯蓣皂苷元的含量和产率,且灭活菌丝的诱导效果要强于菌液浓缩物。Dzf17灭活菌丝处理无菌苗,薯蓣皂苷元的产率为78.697mg/L,是对照(27.471mg/L)的2.865倍;用Dzf17菌液浓缩物处理无菌苗,皂苷元产率为41.822mg/L,是对照的1.522倍。Dzf17灭活菌丝处理培养细胞,薯蓣皂苷元的产率为1.391mg/L,是对照(0.691mg/L)的2.013倍;用Dzf17菌液浓缩物处理培养细胞,皂苷元产率为1.214mg/L,是对照的1.757倍。结果表明,在盾叶薯蓣无菌苗或细胞培养中添加一定量的内生真菌灭活菌丝或菌液浓缩物对于提高薯蓣皂苷元含量和产量将是一种有效的方法。

Nine endophytic fungal isolates were separated from the fresh healthy rhizomes of Dioscorea zingiberensis,and they were taxonomically identified by both morphological characters and r-DNA molecular analysis.Their autoclaved mycelia and concentrated filtrates were prepared after two weeks of suspension culture.Of them,isolate Dzf17,which was identified as Fusarium oxysporum,was found to effectively increase diosgenin content and yield of the seedlings and suspension cells of D.zingiberensis,especially the el...