长春花萜类吲哚生物碱含量测定及相关基因的表达分析

Measurement and Analysis of Important Alkaloids and Related Gene Expression in Catharanthus roseus

采用反相高效液相色谱法(RP-HPLC)对23个长春花品种中的重要萜类吲哚生物碱文多灵、长春质碱和长春碱含量进行了测定,发现这3种生物碱的含量在不同品种中存在较大差异。相关性分析表明,文多灵、长春质碱和长春碱这3种生物碱中,文多灵含量和长春碱含量有极显著的相关性(P<0.01)。利用RT-PCR分析了香叶醇10-脱羧酶基因(g10h)和异胡豆苷合成酶基因(str)在萜类吲哚生物碱含量有显著差异的品种之间的表达水平差异,并结合生物碱含量数据结果,发现g10h和str的表达水平和文多灵和长春质碱的总含量有显著的正相关性(P<0.05),说明g10h和str基因可以作为长春花中文多灵和长春质碱含量的参考基因标记。该研究对为选育高萜类吲哚生物碱含量长春花品种及长春花萜类吲哚生物碱代谢工程具有重要意义。

The contents of three important terpenoid indole alkaloids(TIAs)including vindoline,catharanthine and vinblastine in 23 Catharanthus roseus varieties were measured by Reversed Phase High Performance Liquid Chromatography(RP-HPLC).It was found that the contents of the three alkaloids varied along with the different varieties.Correlation analysis indicated that among the three alkaloids,vindoline and vinblastine contents were correlated at very significant level(P<0.01).RT-PCR was used to analyze the expre...