五种苦苣苔科植物α-葡萄糖苷酶抑制活性研究

α-Glucosidase Inhibitory Activity of Extracts of Five Genera of Gesneriaceae

利用体外α-葡萄糖苷酶抑制模型对5种苦苣苔科植物进行活性评价,并与阳性对照Acarbose进行比较,发现5种植物不同部位均有一定的α-葡萄糖苷酶抑制活性。其中,牛耳岩白菜石油醚部位的抑制活性最高(IC50=26.19μg/mL,活性均远大于阳性对照Acarbose(IC50=1081.27μg/mL)。不同植物比较,牛耳岩白菜的α-葡萄糖苷酶抑制活性最好,其3种不同溶剂提取物与Acarbose相比均有很高抑制活性;对牛耳岩白菜提取物的α-葡萄糖苷酶抑制动力学研究结果表明,石油醚和乙酸乙酯提取物对α-葡萄糖苷酶抑制作用属于非竞争性抑制类型,Ki值分别为4.24和40.04μg/mL。正丁醇提取物则属于竞争性抑制类型(Ki=205.48μg/mL)

The inhibitory activities of α-glucosidase of different extracts from five genera of Gesneriaceae were assayed in vitro and the results were compared with Acarbose.The results showed that different parts of five genera all had activity,and Chirita eburnean Hance had the highest inhibitor activity of α-glucosidase in the five genera.The petroleum ether extract of C.eburnean Hance had the highest inhibitor activity of α-glucosidase(IC50 =26.19 μg/mL),which was lower than that of Acarbose(IC50=1081.27 μg/mL).T...