鱼体内环境激素DMP和DEP的检测方法

Simultaneous Determination of Dimethyl Phthalate (DMP) and Diethyl Phthalate (DEP) in Plasma and Muscle of Crucian carp(Carassuys auratus)

建立同时检测鲫(Carassuys auratus Linnaeus)血浆和肌肉中邻苯二甲酸二甲酯(DMP)和邻苯二甲酸二乙酯(DEP)的高效液相色谱方法,进行初步的代谢动力学研究。血浆样品经液-液萃取,肌肉样品经液-液萃取和固相小柱处理后,以乙腈∶水=40∶60(V/V)为流动相,流速1.0mL/min,采用SHIMADZU ODS柱(150mm×4.6mm;i.d.,5μm)色谱柱分离。测定鲫经口染毒后血浆和肌肉中DMP和DEP的浓度。结果表明,标准曲线线性范围为0.05~10.0μg/mL,线性关系良好(r>0.99),定量限为0.05μg/mL,回收率95%以上,日内变异系数和日间变异系数均低于15%。经口染毒48h内,鲫血浆和肌肉中均能检测到DMP和DEP的残留。该法操作简便、快速、灵敏、准确,已经初步用于鲫血浆和肌肉中DMP和DEP的代谢动力学和残留研究。

To develop an HPLC-UV method for simultaneous determination of dimethyl phthalate(DMP)and diethyl phthalate(DEP)in Crucian carp plasma and muscle.Then the metabolic kinetics was primarily studied.After a simple liquid-liquid extraction and SPE treatment,DMP,DEP and the internal standard were separated on SHIMADZU C18 column(150 mm×4.6 mm,5 μm)and detected by UV spectrometry.The detection wavelength was set at 235 nm.The mobile phase consisted of acetonitrile-water(40∶60)at a flow rate of 1.0 mL/min.The conc...