马铃薯遗传多样性的ISSR分析

Genetic Diversity Analysis Based on Potato ISSR

利用ISSR分子标记分析了20个马铃薯材料的遗传多样性和亲缘关系。从60条ISSR引物中筛选出10个ISSR引物,在供试材料中共扩增出57个清晰条带,其中48个具有多态性,多态性比率为84.21%,各扩增条带的多态性信息指数(PIC)平均为0.2055。种质间遗传相似系数变化范围为0.5263～0.9825,平均值为0.7220。通过聚类分析20份马铃薯材料分为五大类,富金单独是一类,第二类有2个品种,即克新17号和黑美人,大西洋和YN-1为第三大类,克新20号和中薯1号为第四类,其余归到第五大类。试验结果显示,所选马铃薯材料的遗传差异比较大,遗传多样性相对丰富。本研究的结果为马铃薯杂交育种的亲本选配提供了理论依据。

Inter-simple sequence repeat(ISSR)markers were employed to analyze the genetic diversity of 20 potato(Solanum tuberosum) varieties.Fifty-senven DNA fragments were amplified by using 10 primers screened from 60 ISSR primers,of which 48 DNA bands were polymorphic,and the polymorphism ratio was 84.21%.The average polymorphism information content(PIC) was 0.2055.The genetic similarity among different varieties ranged from 0.5263 to 0.9825,with an average of 0.7220.The cluster analysis based on ISSR markers reve...