摘要
The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.
The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.
基金
This work was supported by the National Natural Science Foundation of China(Grant No.30370857)
Major Project of Ministry of Education in China
National 863 High Technology Research and Development Project(No.2001AA241032).
