三七芪连汤治疗萎缩性胃炎有效部位纯化工艺研究

Study on the technology of purification of the effective fractions treated for atrophic gastritis in

目的考察5种大孔吸附树脂对三七芪连汤治疗萎缩性胃炎有效部位———总皂苷和总生物碱的吸附分离性能,确定大孔吸附树脂吸附分离三七芪连汤总皂苷和总生物碱的工艺条件。方法以总皂苷和总生物碱吸附量、含量和回收率为考察指标;总皂苷和总生物碱含量测定、泄露曲线和洗脱曲线考察均采用紫外-可见分光光度法。结果AB-8型大孔吸附树脂对三七芪连汤总皂苷及总生物碱有良好吸附分离性能,适宜的工艺条件为:复方提取物上样浓度为40mg/mL,总皂苷和总生物碱最大吸附量(单位树脂湿体积吸附量)分别为5.28mg/mL和12.38mg/mL,吸附流速为1mL/min,洗脱剂为50%乙醇,洗脱剂用量为8倍量树脂体积,洗脱流速为2mL/min,树脂经95%乙醇和1mol/LNaOH再生后,可重复使用4次。结论AB-8型大孔吸附树脂在所确定的工艺条件下,可较好地吸附分离三七芪连汤总皂苷和总生物碱,乙醇洗脱物中总皂苷含量达20%以上,总生物碱含量达30%以上,总皂苷和总生物碱的回收率分别达75%和65%以上。

Objective To investigated the absorptive and separative properties of 5 kinds of macroporous absorbing resins to the total saponins and alkaloids,the effective fractions which treat atrophic gastritis in Sanqi Qilian Tang. To study the technology of absorption and separation of the total saponins and alkaloids in Sanqi Qilian Tang by macroporous absorbing resin. Methods Selected the absorption capacities, contents and recovery rates of the total saponins and alkaloids as the evaluating indications. The determination of the contents of the total saponins and alkaloids and the investigation to leaking and eluting curve were completed with UV-VIS spectrophotometric method. Results AB-8 resin had the best absorptive and separative properties to the total saponins and alkaloids in Sanqi Qilian Tang. The applicable technology was as follows: the concentration of original solution of the extract of Sanqi Qilian Tang was 40mg/mL; the largest absorption capacities on resin to the total saponins and alkaloids were 5.28mg/mL and 12.38mg/mL respectively; absorbing current velocity was 1mL/min; the eluant was 50% Ethanol with 8 times of resin volume, and the eluting current velocity was 2mL/min. AB-8 resin could be used for 4 times repeatedly after reproducted by 95% Ethanol and 1mol/L NaOH. Conclusion Under the process condition which had been selected above, AB-8 resin can be used successfully to absorb and separate the total saponins and alkaloids in Sanqi Qilian Tang, with over 20% of the content of the total saponins and 30% of the content of the total alkaloids in the 50% Ethanol eluate, and with over 75% of total saponins recovery and 65% of total alkaloids recovery respectively.