摘要
制备灿烂弧菌(Vibrio splendidus)福尔马林灭活菌苗,通过浸泡、注射和口服3种方式接种美国红鱼(Sci-aenops ocellatus),分别在接种免疫后第7、14、21、28、35天采鱼血,检测其血清抗体效价、溶菌酶活力和白细胞吞噬活性;第28天用1.0×108CFU/mL的灿烂弧菌悬液进行攻毒试验,检测菌苗的免疫效果。结果表明,各免疫组血清抗体效价在第21天达到峰值,且浸泡组和注射组的血清抗体效价高于口服组;在35d实验期内,各免疫组的血清溶菌酶活力有明显提高,各浸泡组和注射组于免疫接种后14d显著高于对照组(P<0.05);除浸泡3组外,其余各免疫组的白细胞吞噬百分比(PP)和吞噬指数(PI)在免疫接种后14d均较对照组有显著差异(P<0.05);各组受免鱼对人工攻毒均具有保护作用,以注射2组的免疫保护率最高,达77.8%。
Sciaenops ocellatus were vaccinated with a formalin-killed Vibrio splendidus vaccine via immersion,injection and oral route,respectively. Antibody titers,lysozymes,leucocyte phagocytosis were examined on the 7th, 14th,21st,28th d after first-lvaccination. A dosage of 0. 2 mL of V. splendidus 1. 0 × 108 CFU/mL was intraperitoneally injected to the vaccinated fish to evaluate the protective efficiency of the vaccine. The results indicated that: the antibody titers of all immunized group was the highest on the...
出处
《水生态学杂志》
北大核心
2010年第3期137-141,共5页
Journal of Hydroecology
基金
浙江省科技厅科研院所青年人才计划项目(2006R20001)
浙江省科技厅科研院所专项公益技术攻关项目(2007F10009)
温州市科技兴海项目(S20070051)联合资助
关键词
美国红鱼
灿烂弧菌
菌苗
Sciaenops ocellatus
Vibrio splendidus
Vaccine
