温针灸对糖尿病大鼠坐骨神经AGEsmRNA、RAGEmRNA表达的影响

The Influence of Warm Needling on SOD and MDA of Blood Serum and Sciativ Nerve in Diabetic Peripheral Neuropathy Modle Rats

目的:探讨温针灸法对糖尿病周围神经病变(DPN)模型大鼠氧化应激水平的影响。方法:本课题采用体重在200 g左右的雄性Wistar大鼠40只,选其中30只以(STZ)左下腹单次注射诱导形成糖尿病模型,造模后选血糖≥16.7 mmol/l的Wistar大鼠喂养8周形成DPN模型。随机分为模型对照组、弥可保组、温针组,其余10只为正常对照组,每组10例。温针组选用脾俞、肾俞、足三里、环跳等穴位进行针刺,得气后,在针尾上搓捏艾绒点燃施灸。每穴施灸20 min,隔日1次,共治疗8周。弥可保组肌注弥可保50μg/kg。通过对大鼠一般情况、血糖监测,结合神经电生理方法检测坐骨神经传导速度,评价温针对DPN的治疗效应。以免疫组织化学法检测坐骨神经AGEs,用RT-PCR检测坐骨神经RAGEmR-NA的表达。结果:治疗8周后,温针治疗提高了DPN大鼠坐骨神经传导速度,抑制坐骨神经中AGEs及RAGEmRNA的表达,其结果与弥可保组比较有显著性差异(P<0.05)。结论:本课题证实了温针疗法可以提高DPN模型大鼠的坐骨神经传导速度,能抑制坐骨神经AGEs及RAGEmRNA的表达,减少AGEs与RAGE结合产生的生物学效应,从而保护神经细胞免受氧化应激损伤,减轻了对神经和血管的损害,进而改善了周围神经的结构和功能,这可能是其治疗DPN的作用机制之一。

Objective:To research the influence on level of oxidize stress of diabetic peripheral neuropathy model rats by warm needling.Methods:This issue use male Wistar rat with weight some 200g,The rats once injected with streptozotocin on left infeior belly in one time were induced to diabetic model,then feed the rats with the blood sugar over 16.7mmol/l 8 weeks to DPN model.Randomly divided these rats into model constract group,acqupuncturing group,warm needling group and nomal group separately,each group involve...