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Epigenetic Regulation of the ERβ Gene on the Estrogen Signal Transfection Pathway in Colon Cancer Cells 被引量：1

Epigenetic Regulation of the ERβ Gene on the Estrogen Signal Transfection Pathway in Colon Cancer Cells
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摘要 We studied the regulatory effects of the estragen receptorβ(ERβ)gene on the downstream estrogen signal transfection pathway in colon cancer cells and the possible mechanisms involved.A human ERβ gene recombinant expression plasmid,pEGFP-C1-ERβ,was constructed and transfected into the Caco-2 colon cancer cell line,a line with low ERβ gene expression.The expression of ERβ mRNA and protein was detected 72h after transfection.RT-PCR was used to examine the expression levels of the progesterone recepror(PR)gene ... We studied the regulatory effects of the estragen receptorβ(ERβ)gene on the downstream estrogen signal transfection pathway in colon cancer cells and the possible mechanisms involved.A human ERβ gene recombinant expression plasmid,pEGFP-C1-ERβ,was constructed and transfected into the Caco-2 colon cancer cell line,a line with low ERβ gene expression.The expression of ERβ mRNA and protein was detected 72h after transfection.RT-PCR was used to examine the expression levels of the progesterone recepror(PR)gene ...
作者 翟荣林 王国斌 蔡开琳 陶凯雄 许飞 张万里 王智勇
机构地区 Department of General Surgery
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2010年第1期69-74,共6页 华中科技大学学报（医学英德文版）
基金 supported by a grant from the National Natural Sciences Foundation of China(No.30500488)
关键词 estrogen receptor β signal transfection methylation colon cancer EPIGENETICS estrogen receptor β signal transfection methylation colon cancer epigenetics
分类号 R735.35 [医药卫生—肿瘤]
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参考文献10

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同被引文献9

  • 1Jones PA, Baylin SB. The epigenomics of cancer [J]. Cell, 2007, 128(4) : 683-692.
  • 2Esteller M. Dormant hypermethylated tumor suppressor genes: questions and answers [J]. J Pathol, 2005, 205(2) : 172-180.
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  • 6Menigatti M, Cattaneo E, Sabates-Bellver J, et al. The protein tyrosine phosphatase receptor type R gene is an early and frequent target of silencing in human colorectal tumorigenesis [ J]. Mol Cancer, 2009, 8 : 124.
  • 7Krishnan M, Singh AB, Smith JJ, et al. HDAC inhibitors regulate claudin-1 expression in colon cancer ceils through modulation of mRNA stability [J]. Oncogene, 2010, 29(2) : 305-312.
  • 8Kim M, Louwagie J, Carvalho B, et al. Promoter DNA methylation of oncostatin m receptor-beta as a novel diagnostic and therapeutic marker in colon cancer [J]. PLoS One, 2009, 4(8) : e6555.
  • 9Hibi K, Mizukami H, Shirahata A, et al. Aberrant methylation of the netrin-1 receptor genes UNC5C and DCC detected in advanced colorectal cancer [J]. World J Surg, 2009, 33(5): 1053-1057.

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Journal of Huazhong University of Science and Technology(Medical Sciences)
2010年 第1期

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