高效液相色谱法同时测定烟用水基胶中的甲醛和乙醛

Determination of formaldehyde and acetaldehyde in cigarette adhesives by high performance liquid chromatography

建立了用高效液相色谱法同时测定卷烟胶中甲醛、乙醛的分析方法。卷烟胶用去离子水提取,在酸性条件下,经2,4-二硝基苯肼(DNPH)衍生后,衍生物直接用HPLC法检测。结果表明:该方法的标准曲线在相关浓度范围内呈良好线性关系,甲醛、乙醛的检出限分别为3.6、6.2μg/L,阶醛、乙醛的样品回收率分别为98.38%~103.11%,89.37%~90.12%,相对标准偏差均小于6%。

A method for the simultaneous determination of formaldehyde and acetaldehyde in cigarette adhesives was established using high-performance liquid chromatography with a PAD detector.After formaldehyde and acetaldehyde in the samples were extracted with deionized water,the solution reacted with 2,4-dinitrophenylhydrazine in an acidic medium to form 2.4-dinitrophenylhydrazone,which were separated on the thermo-C18 column(150 mm×4.6 mm,5 μm) directly.Good linearities were obtained over corresponding concentrati...