卤代荧光素与西布曲明的荧光猝灭和褪色光谱及其分析应用

Study on fluorescence quenching and fading spectra of halofluorescein dyes and sibutramine and their analytical applications

在弱酸性缓冲溶液中,乙基曙红(EE)、赤藓红(ET)和荧光桃红(PX)3种卤代荧光素与盐酸西布曲明(SH)形成离子缔合物,导致吸收光谱发生变化和荧光猝灭。研究了反应产物的吸收和荧光光谱特征,适宜的反应条件,据此建立了以卤代荧光素为光谱探针的灵敏、简便、快速测定SH的新方法。其中ET体系褪色反应灵敏度最高,对SH的线性范围为0.1～4.0μg/mL,检出限为0.06μg/mL;PX-SH体系的荧光猝灭法对SH的线性范围是0.2～4.6μg/mL(λex/λem=540nm/560nm),检出限为0.09μg/mL。讨论了离子缔合反应对荧光及吸收光谱的影响及卤代荧光素荧光猝灭和褪色的原因。

In weak acidic buffer solutions,3 halofluorescein dyes,such as ethyl eosin (EE),erythrosine (ET) and phloxine (PX),can react with sibutramine (SH) to form ion-association complexes,which not only results in the quenching of fluorescence,but also results in the great decrease of absorbance.In this work,the spectral characteristics of fluorescence and absorption spectra,the optimum conditions of the reaction were investigated.A new method for the determination of sibutramine by using halofluorescein as a prob...