热激诱导不结球白菜热激蛋白合成与耐冷性分析

Induction of heat shock protein synthesis and chilling tolerance in Brassica campestris ssp.chinensis

以不结球白菜暑绿为试材,研究了热激处理(42℃)诱导不结球白菜热激蛋白(BcHSP)合成与耐冷的相关性。结果表明:热激处理明显增强了不结球白菜幼苗的耐冷性,降低了膜伤害程度。超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性含量增加,而丙二醛(MDA)含量下降。已从热激诱导的不结球白菜中克隆了小分子量BcHSP基因(日本DNA数据库DDBJ登录号:AB367955)。荧光实时定量PCR分析表明BcHSP的表达量与耐冷性相一致。低温(4℃)胁迫下,在大肠杆菌中异源表达BcHSP可维持大肠杆菌较高的细胞活力,这些结果表明BcHSP合成与不结球白菜耐冷性的提高有关。

The relationship between heat shock protein expression and plant chilling tolerance was studied in Brassica campestris ssp.chinensis Shulü .Heat shock(42 ℃)applied to Brassica campestris ssp.chinensis seedling increased the chilling tolerance of seedling and decreased the permeability of cellular membranes.The activities of superoxide dismutase,catalase and peroxidase increased,and in contrast,the malondialdehyde content depressed.A full length cDNA of small heat shock protein(sHSP)was isolated from Brassic...