HPLC测定马尿泡不同部位中的东莨菪碱和阿托品

Determination of scopolamine and atropine in different parts of Przewalslia tangtuica Maxim by HPLC

目的采用RP-HPLC法测定马尿泡不同部位中东莨菪碱和阿托品的含量。方法采用Shimadzu VP C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(30∶70,含0.02mol·L-1醋酸钠、0.1%三乙胺、0.3%四氢呋喃、冰醋酸调pH6.48),流速1.0mL·min-1,检测波长215nm,柱温30℃。结果东莨菪碱0.068～0.408μg与峰面积线性关系良好(r=0.9996);阿托品2.498～14.988μg与峰面积线性关系良好(r=0.9999)。结论所用方法简便、准确、灵敏度高、重复性好。

OBJECTIVE To develop a quantitative analytical methods of scopolamine and atropine in different parts of Przewalslia tangtuica Maxim using RP-HPLC.METHODS The content of galic acid was detected by HPLC.The separation was carried out on a Shimadzu VP C18 column(250 mm × 4.6 mm,5 μm).The mobile phase was methanol-0.02 mol·L -1sodium acetate buffer (30∶70,containing 0.1% triethanolamine and 0.3% Tetrahydrofuran and the pH was adjusted to 6.48 with acetic acid).The flow rate was 1.0 mL·min -1.The detective wave...