摘要
目的比较2种登革热抗体ELISA检测试剂盒以及1种ICT(免疫层析法)试剂盒对于登革热抗体检测的差异,筛选出更适合群体血清流行病学检测的试剂盒。方法按照所选择的试剂盒的说明,用盲法对出入境人员血清进行检测。结合检测和流调结果进行分析。结果登革热IgG阳性检出率分别是NOV16.67%、中山10.12%、Cotez0.6%,总体上检出率差异有统计学意义(P<0.05),中山和NOV之间检出率差异没有统计学意义(P>0.05);登革热IgM阳性检出率分别是NOV0%、中山1.19%、Cotez3.57%,三者检出率差异均有统计学意义(P<0.05)。分析三种试剂检测阳性与黄热、乙脑接种、病史的关系,提示Cortez-IgG呈现弱相关趋势。检测成本测算,中山试剂成本最低。结论对于开展人群登革热血清流行病学调查和监测,中山-IgG试剂相对较优。不同检测原理、不同工艺的试剂盒,其检测特性需要进一步研究和评估。
Objective To find out a suitable reagent for dengue seroepidemiology,two ELISA reagents(Novatec and zhongshan)and ICT reagent(Cortez)were applied to detect dengue antibody.Methods According to reagent manual,blood samples collected randomly from entry-exit population were detected by 3 kinds of reagent.Then data from detecting were analyzed.Results The IgG positive rate were 16.67%(NOV),10.12%(zhongshan),0.6%(Cortez)respectively.There was statistical difference between 3 kinds of reagent(P<0.05).But ther...
基金
国家质检总局课题:2006IK184
关键词
登革热
抗体
检测试剂
Dengue
Antibody
Detection reagent
