大鼠肾脏机械损伤早期TNF-α及其受体的表达

The Expression of TNF-α and Acceptor in Renal Early Mechanical Trauma of Rat

探讨外源性ADM对肾脏机械性损伤早期TNF-α及受体表达的影响。选健康成年普通级Wistar大鼠104只,随机分为四组:对照组8只、创伤组32只、创伤前注射ADM组32只、创伤后注射ADM组32只,后三组的动物分别于创伤后1、6、12和24 h各处死8只。采用自由落体打击仪直接打击大鼠脊肋区制作肾机械性损伤模型。ADM分别于创伤前或创伤后10 min采用腹腔注射法给药。实验动物采用快速心脏采血法处死。取下肾脏标本,10%甲醛固定。观察TNF-α、TNFR在组织中的表达规律。结果表明:单纯创伤组于创伤早期TNF-α表达高于正常对照组,外源性ADM早期(1 h和6 h点)可抑制TNF-α(P<0.05)。单纯创伤组于创伤早期(1 h和6 h点)TNFR表达低于正常对照组,外源性ADM(创伤前给药)早期(1 h和6 h点)可上调节TNFR(P<0.05)。说明TNF-α在创伤早期即参与了肾组织炎症的急性期反应并加重损伤。TNFR对TNF-α具有动态调节作用。ADM能降低TNF-α的分泌。ADM在早期肾损伤过程中可以上调TNFR,与其相互协调,共同发挥对损伤器官的保护作用。

To investigate the effects of TNF-α and the acceptor expression about mechanical renal trauma with extragenous ADM.There were 104 healthy adult plain grade Wister rat were randomly divided into four groups:8 in the control group,32 in the trauma group,32 in the group injected ADM before trauma,32 in the group injected ADM post trauma.The 8 rats in the last three groups were excuted at 1,6,12 and 24 h post trauma respectively.The experimental model of rat kidney with mechanical trauma were prepared by striki...