摘要
为了探讨束缚+温水(36±1)℃应激是否能引起大鼠胃粘膜损伤、是否对延髓和下丘脑Fos蛋白表达有影响,本研究将雄性Wistar大鼠随机分两组:实验组,束缚+温水(36±1)℃应激1 h;对照组,室温下单纯束缚应激1 h。应激后处死,测直肠温度;取胃,观察胃粘膜损伤程度;取脑,应用免疫组化染色方法观察两组动物延髓、下丘脑神经元的Fos蛋白表达。结果显示:两组动物胃粘膜损伤程度均较轻或基本无胃粘膜损伤,延髓和下丘脑各核团Fos蛋白表达均无显著性差异,直肠温度也无显著性差异。这些结果提示,由于束缚+温水(36±1)℃应激不改变动物的体温,因而不引起延髓和下丘脑控制胃机能的核团神经元活动加强,从而也不诱发急性胃粘膜损伤。
To investigate the effect of restraint warm water-immersion stress on the Fos expression in medulla and hypothalamus of rat,male Wistar rats weighting 150~200 g were randomly divided into two groups: experimental group,control group.The experimental group was restrained and immersed in warm water(36±1)℃;the control group was only restrained under the room temperature.One hour after stress,the rats were sacrificed.The rectum temperature was measured,the gastric mucosal ulcer index was determined according to...
出处
《生物医学工程研究》
2009年第4期259-262,共4页
Journal Of Biomedical Engineering Research
基金
国家自然科学基金资助项目(30770277)
国家自然科学基金资助项目(30970354)
关键词
束缚+冷水应激
束缚+温水应激
FOS蛋白
延髓
下丘脑
Restraint cold-water-immersion stress
Restraint warm-water-immersion stress
Fos
Medulla
Hypothalamus