灰霉病菌抗感腐霉利菌株形态和生理生化特性的比较

Comparison in morphological,physiological and biochemical feathers between the resistant mutants and the sensitive strain of Botrytis cinerea to procymidone

经紫外线照射、亚硝基胍处理和药剂诱导,分别从灰霉病菌野生敏感菌株XY6-1S获得高抗腐霉利菌株XY6-1R052、XY6-1R140和XY6-1R202,其EC50>100μg.mL-1、MIC>1 000μg.mL-1。在1~4μg.mL-1药剂浓度下,敏感菌株菌丝呈现不同程度的形态异常,甚至细胞壁破损,而抗性菌株菌丝未有明显改变。在含20μg.L-1以上蔗糖或1.25 g.L-1以上NaCl培养基上,抗药菌株菌丝生长明显差于敏感菌株,表明前者比后者对高渗透压敏感。另外,抗药菌株菌丝相对电导率显著高于敏感菌株,显示抗药菌株细胞膜透性明显改变,因而导致电介质渗漏。在含药培养液中,敏感菌株菌丝甘油含量急剧上升,提高904.63%,而抗药菌株菌丝甘油含量仅增加20.54%~44.00%,因此对于高渗环境,敏感菌株具有较好的调节能力。

Three resistant mutants XY6-1R052,XY6-1R140,XY6-1R202 to procymidone were obtained from the sensitive wild strain XY6-1S of Botrytis cinerea treated with ultraviolet,N-methyl-N-nitroguanidine and procymidone respectively.They had high resistance with EC50 of >100 μg·mL-1 and MIC of >1 000 μg·mL-1.Procymidone at the concentration of 1-4 μg·mL-1 could not only hinder the mycelial growth of the sensitive strain,but also lead to the hyphal distortion and cell wall disruption.But there were no distinct eff...