断奶仔猪腹泻病原PCR鉴定及其药敏试验

PCR detection and antibiotic sensitive test of the pathogen responsible for porcine postweaning diarrhea

江苏某养殖场断奶仔猪发生水样腹泻,使用庆大霉素治疗无效。通过采集其中3例腹泻病猪的肛拭子样品,接种LB肉汤培养6 h后,用PCR方法检测,结果发现仔猪腹泻由产肠毒素大肠杆菌(ETEC)引起。通过细菌分离获得60株细菌,PCR检测结果表明:其中2株为LT1+ST2型ETEC、1株为ST1型ETEC,且其菌毛型均为F18ac;药敏试验结果表明:这3株细菌均对呋喃妥因高度敏感,对多黏菌素中度敏感,对环丙沙星、新霉素低度敏感,对四环素、庆大霉素耐药,对左氟沙星、头孢唑林因不同菌株也有差异。另外,在肛拭子样品中还发现大量小袋纤毛虫,选用呋喃妥因和甲硝唑治疗,所有腹泻病猪均于5 d后康复。

Some postweaning piglets were suffered from watery diarrhea in a wild boar farm in Jiangsu provice,China,and it was no effect after using gentamicin.The rectal swab samples were obtained from three live diarrheic piglets,and were diagnosed being infected with enterotoxigenic Escherichia coli(ETEC) by rapid detected PCR method described previously.In addition,60 bacteria isolates were picked from all the 3 samples,and three F18ac-positive ETEC isolates were picked out and determined by PCR examination,therei...