摘要
将120只600日龄产蛋山麻鸭随机分为正常日粮组、1/2石粉组和1/4石粉组,自动生化分析仪测定更换饲料前1 d及更换饲料后1、3、5周血清钙、磷水平,观察缺钙日粮对蛋鸭血清钙、磷的影响。分离山麻鸭破骨细胞(OC),在此基础上添加不同浓度钙离子(2、3、57、mmol.L-1),倒置显微镜观察体外培养OC形态,培养1、3、57、d进行抗酒石酸酸性磷酸酶染色(TRAP),7 d后扫描电镜观察象牙片吸收陷窝。结果表明:钙缺乏可导致蛋鸭血清钙、磷水平升高,降低钙磷比例。高浓度钙和较低浓度钙显著抑制OC生成及骨吸收活性。结果说明缺钙可诱导OC生成并发挥骨吸收功能,最终导致骨代谢性疾病。
To investigate the effects of serum calcium and phosphorus on the model of calcium-deficient laying ducks,one hundred and twenty Shan sheldrakes were allocated into normal diet,half calcium carbonate and quarter calcium carbonate groups randomly.Serum calcium and phosphorus were detected by automatic biochemistry analyzer one day before and one,three and five weeks after fed with above diets.Osteoclasts(OC) isolated from Shan sheldrakes were cultured with different concentrations of calcium(2,3,5,7 mmol·L-1...
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第4期19-22,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(30571364
309722229)
江苏省自然科学基金资助项目(BK2009736)
关键词
鸭
钙
磷
破骨细胞
抗酒石酸酸性磷酸酶
duck
calcium
phosphorus
osteoclast
tartrate resistant acid phosphatase
