超高效液相色谱-串联四极杆质谱法测定水产品中氯霉素残留量

Determination of chloramphenicol residues in acquatic products by ultra performance liquid chromatography-electrospray tandem mass spectrometry

建立超高效液相色谱-串联四极杆质谱法测定水产品中氯霉素残留量的方法。样品均质后经乙酸乙酯提取,正己烷脱脂(或用固相萃取柱净化),超高效液相色谱分离,串联四级杆质谱检测,同位素内标法定量。方法在0.05～1.0μg/kg的添加范围内的平均回收率为84.9%～103.3%,相对标准偏差为3.2%～5.2%,定量检测限为0.02μg/kg。方法适用于各种水产品基质的氯霉素残留检测。

An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed to determine the residue of chloramphenicol in aquatic products.The samples,labled by the isotopically internal standard,were extracted by ethyl acetate,then defatted with n-hexane(or purified by SPE).Chromatographic separation was achieved by using UPLC,and the detection was performed by mass spectrometry.Results showed that the recoveries for chloramphenicol at the spiked concentrations of 0.05～1.0 μg/kg...