基于中性红电聚合膜的伏安型甲胎蛋白免疫传感器

A voltammetric α-fetoprotein immunosensor based on electropolymerized neutral red film

用循环伏安法在玻碳电极上电聚合一层稳定的中性红聚合物膜,再通过共价键合作用将戊二醛和甲胎蛋白抗体自组装到电极表面,最后用辣根过氧化物酶封闭电极上的非特异性吸附位点,并同时起到放大响应电流信号的作用,制得高灵敏伏安型甲胎蛋白抗原免疫传感器。实验结果表明,该传感器对甲胎蛋白抗原具有良好的电流响应,该传感器的线性范围为1.00～10.0ng/mL和10.0～200ng/mL,检出限(3σ)为0.40ng/mL。

A voltammetric α-fetoprotein immunosensor was constructed by using an electropolymerized neutral red (PNR) film on a glassy carbon electrode. A high density of amino groups of PNR was used to covalently attach protein probes via the aid of glutaraldehyde cross-linking,leading to significantly improved detection stability compared with conventional entrapment methods. HRP was used instead of bovine serum albumin to block unspecific sites,and the amperometric response signal was amplified,thus the sensitivity...