反相高效液相色谱手性固定相法分离喹禾灵光学异构体

Enantioseparation of Quizalofop-ethyl on Chiral Stationary Phase by RP-HPLC

对除草剂喹禾灵光学异构体进行有效的分离。以Chiralcel AD-RH手性柱的R-HPLC,分别考察了流动相乙腈-水的比例、流速及柱温对喹禾灵光学异构体手性分离的影响。确定了分离喹禾灵光学异构体的最佳色谱条件:流动相为乙腈-水(体积比65∶35),流速为0.5mL/min,柱温为25℃。在所选定的色谱条件下,喹禾灵光学异构体的分离度(R)和分离因子(α)分别为2.11和1.19。并通过分离过程中的热力学参数的计算,探讨Chiralcel AD-RH手性柱对喹禾灵光学异构体的手性识别机理,证实了喹禾灵光学异构体的分离是焓驱动的过程。

Study was separated from quizalofop-ethyl optical isomers of the best ratio of mobile phase,flow rate and column temperature and other parameters,and chiral recognition mechanism was discussed.The experimental results showed that the optimum separation conditions of optical isomers of quizalofop-ethyl are as follows:CH 3 CN-H 2 O(65:35, by vol)as mobile phase at a flow rate of 0.5 mL/min,the column temperature 25°C.Under the selected conditions,the separation degree and separation factor of optical isomers ...