摘要
目的构建稳定表达SirT7的P19细胞系并探讨Ⅲ类组蛋白去乙酰基酶SirT7在该细胞中对细胞增殖和细胞周期的影响。方法将SirT7的真核表达质粒转染进入P19细胞,并利用筛选标记筛选出稳定克隆,采用Western blot、流式细胞仪等方法观察SirT7对P19细胞生长和细胞周期的影响。结果稳定表达Sirt7 P19细胞系细胞生长速度减慢,流式细胞仪荧光分析呈现明显的G1/S期阻滞。结论SirT7可导致P19细胞的周期阻滞,并抑制细胞的增殖。
Objective To study the regulatory role of SirT7,one of class Ⅲ histone deacetylases,on the proliferation of mouse embryonal carcinoma cell line P19.Methods We used an expression plasmid of SirT7(151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines.Recombinant DNA techniques,Western blot,cell growth curve,and flow cytometry were used in this paper.Results Compared with the control cells,the P19 cells had significantly lower growth rate ...
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2009年第6期724-727,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(30721063)
国家(重点)实验室专项基金(2060204)~~
