PIH1D1对染色质重塑复合物SNF5亚基降解的影响

Effect of PIH1D1 on the Degradation of Its Binding Protein SNF5

目的探讨PIH1D1对其结合蛋白SNF5的稳定性的影响。方法通过蛋白合成抑制剂环己酰亚胺(CHX)和蛋白酶体抑制剂MG132抑制作用找到SNF5的降解路径,而后转染PIH1D1真核表达质粒,探讨其对SNF5稳定性的影响。结果得到CHX和MG132的最佳作用浓度;SNF5通过蛋白酶体依赖的途径降解;PIH1D1抑制SNF5的降解,从而稳定SNF5。结论PIH1D1可能通过阻断蛋白酶体依赖途径,抑制SWI/SNF染色质重塑复合物SNF5亚基的降解,从而起到稳定SNF5的作用。

Objective To explore the effects of PIH1D1 on its binding protein SNF5,a core subunit of the SWI/SNF chromatin remodeling complex.Method The degradation pathway of SNF5 was identified with protein synthesis inhibitor cycloheximide(CHX) and a potent proteasome inhibitor MG132,and then the PIH1D1 eukaryotic expression plasmid was transfected to explore its effect on the stability of SNF5.Results HEK293T cells were effectively treated with CHX(optimal concentration: 400 μg/ml) and MG132(optimal concentration: ...