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PIH1D1对染色质重塑复合物SNF5亚基降解的影响 被引量:1

Effect of PIH1D1 on the Degradation of Its Binding Protein SNF5
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摘要 目的探讨PIH1D1对其结合蛋白SNF5的稳定性的影响。方法通过蛋白合成抑制剂环己酰亚胺(CHX)和蛋白酶体抑制剂MG132抑制作用找到SNF5的降解路径,而后转染PIH1D1真核表达质粒,探讨其对SNF5稳定性的影响。结果得到CHX和MG132的最佳作用浓度;SNF5通过蛋白酶体依赖的途径降解;PIH1D1抑制SNF5的降解,从而稳定SNF5。结论PIH1D1可能通过阻断蛋白酶体依赖途径,抑制SWI/SNF染色质重塑复合物SNF5亚基的降解,从而起到稳定SNF5的作用。 Objective To explore the effects of PIH1D1 on its binding protein SNF5,a core subunit of the SWI/SNF chromatin remodeling complex.Method The degradation pathway of SNF5 was identified with protein synthesis inhibitor cycloheximide(CHX) and a potent proteasome inhibitor MG132,and then the PIH1D1 eukaryotic expression plasmid was transfected to explore its effect on the stability of SNF5.Results HEK293T cells were effectively treated with CHX(optimal concentration: 400 μg/ml) and MG132(optimal concentration: ...
出处 《中国医学科学院学报》 CAS CSCD 北大核心 2009年第6期756-759,共4页 Acta Academiae Medicinae Sinicae
基金 国家自然科学基金(30721063) 国家(重点)实验室专项基金(2060204)~~
关键词 SWI/SNF染色质重塑复合物 SNF5 PIH1D1 蛋白酶体依赖降解 SWI/SNF chromatin remodeling complex SNF5 PIH1D1 proteasome pathway
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参考文献10

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同被引文献7

  • 1Kakihara Y, Saeki M. The R2TP chaperone complex : its in-volvement in snoRNP assembly and tumorigenesis[ J]. Bio-mol Concepts, 2014, 5: 513-520.
  • 2Horejsi Z,Takai H, Adelman GA, et al. GK2 phospho-de-pendent binding of R2TP complex to TEL2 is essential formTOR and SMG1 stability [ J ]. Mol Cell, 2010,39:839-850.
  • 3Kakihara Y, Houry WA. The R2TP complex : discovery andfunctions [ J ]. Biochim Biophys Acta, 2012,1823:101-107.
  • 4von Morgen P, Horejsi Z, Macurek L. Substrate recognitionand function of the R2TP complex in response to cellularstress [ J ]. Front Genet, 2015,6: 69.
  • 5Yoshida M, Saeki M, Egusa H, et al, RPAP3 splicing va-riant isoform 1 interacts with PIH1D1 to compose R2TPcomplex for cell survival [ J ]. Biochem Biophys Res Com-mun, 2013,430: 320-324.
  • 6Boulon S, Pradet-Balade B, Verheggen C, et al. HSP90and its R2TP/Prefoldin-like cochaperone are involved in thecytoplasmic assembly of RNA polymerase II [ J ] . Mol Cell,2010,39: 912-924.
  • 7Inoue M, Saeki M, Egusa H,et al. PIH1D1,a subunit ofR2TP complex,inhibits doxorubicin-induced apoptosis[ J].Biochem Biophys Res Commun, 2010, 403 : 340-344.

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