酶解法转化人参皂苷Rh_1及其含量测定

Determination and enzymolysis preparation of ginsenoside Rh_1

目的:采用酶解法水解原人参皂苷三醇组获得次级皂苷20(S)-Rh1。为大量制备稀有皂苷20(S)-Rh1提供理论依据。方法:采用AB-8大孔树脂法分离人参茎叶总皂苷提取物,得到原人参皂苷三醇组总皂苷(主要含Rg1和Re)。利用蜗牛酶水解原人参皂苷三醇组,得到次级人参皂苷20(S)-Rh1。建立高效液相色谱分析方法对其进行转化率测定。色谱条件:YMC C18柱(4.6 mm×150 mm,5μm);柱温25℃;检测波长203 nm;流速1 mL.min-1;流动相乙腈(A)-水(B)梯度洗脱,0~29 min,19%~26%A;29~30 min,26%~30%A;30~55 min,30%~38%A;55~60 min,38%~40%A。结果:采用AB-8大孔树脂可以获得高纯度的原人参皂苷三醇组。酶解平均转化率为36.7%,高效液相色谱法可靠、稳定。结论:本方法可以高效率的获得次级皂苷20(S)-Rh1。进而拓展了稀有皂苷20(S)-Rh1的制备来源。

Objective:To obtain 20(S)-ginsenoside Rh1 by the method of enzymolysis with the protopananxtriol saponins,and to provide the theory for large-scale preparation of 20(S)-ginsenoside Rh1.Method:AB-8 macroporous resin was used to isolate the total saponins of the stems and leaves from Panax ginseng and the protopanaxtriol saponins(mainly included Rg1 and Re) were obtained.Then,we used enzymic hydrolysis(helicase)with the protopanaxtriol saponins to get the secondary ginsenoside 20(S)-Rh1.High performance liqui...