京尼平苷酸对佐剂性关节炎大鼠抗炎作用及滑膜细胞凋亡机制研究

Study of geniposide-acid on anti-inflammatory action for adjuvant-induced arthritis rats and mechanism of synoviocyte apoptosis in vitro

目的:研究京尼平苷酸(GA)对佐剂性关节炎(AA)大鼠抗炎作用,探讨GA对AA大鼠滑膜细胞增殖影响的可能凋亡机制。方法:Wistar大鼠随机分为6组,分别为正常组,模型组,200,100,50 mg.kg-1GA组和0.75 mg.kg-1甲氨蝶呤(MTX)组,右后足趾皮内注射弗氏完全佐剂后第19天给药,治疗4周,测定足肿胀指标和血清肿瘤坏死因子-α(TNF-α),白介素-1β(IL-1β)水平。培养AA大鼠滑膜细胞给予GA(1,2,4μmol.L-1)和4μmol.L-1MTX处理,MTT法检测细胞增殖,Ho-echst/PI双染观察细胞凋亡,流式细胞仪检测凋亡细胞率,RT-PCR法检测Bcl-2和Bax基因mRNA表达水平。结果:200,100mg.kg-1GA可以明显降低足肿胀度和关节炎指数(P<0.05或P<0.01),肿胀抑制率分别为25.4%,21.37%,并抑制血清中TNF-α,IL-1β水平(P<0.05),TNF-α抑制率为34.61%,28%,IL-1β抑制率为29.05%,21.65%。GA(1~4μmol.L-1)处理AA大鼠滑膜细胞5 d,明显抑制细胞增殖。流式细胞仪检测显示1,2,...

Objective:To study the effect of geniposide-acid(GA) on the anti-inflammatory action for adjuvant-induced arthritis(AA) rats and the proliferation of synoviocytes in AA rats and the feasible mechanism of apoptosis in vitro.Method: Forty-eight health male Wistar rats were divided randomly into six groups and were administered respectively with 200,100,50 mg·kg-1 GA and 0.75 mg·kg-1 MTX and normal sodium(normal or model control group) for four weeks when right posterior paw pads of rats excluding normal contr...