小续命汤有效成分组中防己诺林碱和粉防己碱在大鼠体内药动学研究

Pharmacokinetics of fangchinoline and tetrandrine in rats

目的:对防己诺林碱与粉防己碱两种化合物在大鼠体内的药动学及复方给药的影响进行研究。方法:采用高效液相色谱-质谱联用技术,建立了防己诺林碱与粉防己碱的血药浓度检测方法,并对单体给药与小续命汤有效成分组给药两种情况下两个化合物的药动学参数进行对比。结果:与有效成分组给药相比,单体给药防己诺林碱与粉防己碱的Tmax显著延长,其余参数没有大的差异。结论:有效成分组中其他成分减慢了二者在体内的吸收速度,与单体给药相比,有效成分组给药时防己诺林碱与粉防己碱的生物利用度不变。

Objective:To investigate the pharmacokinetics profile of fangchinoline and tetrandrine in rats after administration of single compound and mixture with other intergradient in traditional prescription.Method: A method for determination of fangchinoline and tetrandrine in rat plasma by using HPLC-MS has been developed and validated.The pharmacokinetics of two compounds and two compounds in the effective component group(ECG) of Xiaoxuming decoction were compared.Result: Compared with the single dose of compoun...