水分胁迫条件下不同栽培居群菘蓝中4种有机酸的变化

Effect of water stress on content of four organic acids in different cultivated populations of Isatis indigotica

目的:检测北京、内蒙古、河北和陕西等4个栽培居群菘蓝叶片中草酸、苹果酸、柠檬酸和抗坏血酸等4种有机酸成分含量的差异及在淹水胁迫下的变化。方法:采用高效液相色谱法测定正常及水分胁迫下不同栽培居群的菘蓝叶片中4种有机酸的含量。结果:不同栽培居群叶片中有机酸含量不同,而在水分胁迫条件下,各栽培居群有机酸含量变化趋势相同,草酸、苹果酸和柠檬酸含量升高,抗坏血酸含量下降。结论:不同栽培居群的菘蓝所含各有机酸存在一定差异,在水淹等逆境条件下,其叶片中有机酸含量发生不同程度地变化,并与其逆境生理密切相关。

Objective: To study the different and the variety of four organic acids in leaves of Isatis indigotica among different cultivated populations in water stress condition and healthy plant.Method: Four kinds of organic acids,such as oxalic acid,malic acid,ascorbic acid and citric acid from the leaves,were detected by HPLC.Result: Significant differences of four organic acids in the leaves of Beijing,Mongolia,Hebei and Shanxi cultivated populations.Compared with the healthy plant,the content of oxalic acid,mali...