丹皮酚体外黏膜渗透性及影响因素的考察

Study on in vitro mucosal permeation properties and factors affecting mucosal permeability of paeonol

目的:考察牡丹皮提取物丹皮酚的体外黏膜渗透性及影响因素。方法:选择离体牛蛙皮肤为渗透模型,采用改良的Franz扩散池进行体外经皮吸收实验;HPLC测定丹皮酚的含量,研究接收液、蛙皮及药物浓度对丹皮酚体外黏膜渗透的影响。结果:接收液的渗透压和pH对丹皮酚的渗透有一定的影响;同一牛蛙不同部位的皮肤对丹皮酚渗透的影响较大,其表观渗透系数:腹皮>背皮>侧腹皮;丹皮酚黏膜渗透性良好,累积渗透率与时间符合一级吸收过程。结论:以离体牛蛙腹皮为渗透模型,丹皮酚的体外渗透性质与黏膜吸收的特点有相似性。

Objective:To study the in vitro mucosal permeation properties of paeonol and the factors that affect such permeability.Method:Bullfrog skin was selected as the permeation model,and the modified Franz diffusion cell was adopted to investigate the percutaneous adsorption in vitro.Content of paeonol was quantified by HPLC.Kinds of receiving solution,parts of bullfrog skin and impacts of drug concentration on percutaneous adsorption in vitro were systematically studied.Result:Percutaneous permeation of paeonol ...