摘要
目的:研究明党参的遗传多样性,为明党参种质资源利用提供理论依据。方法:以10个不同居群的明党参为材料,通过SRAP分析,计算各样品间的遗传相似系数,在此基础上采用UPGMA方法进行聚类,构建树状图。结果:从160个引物组合中筛选得到17个多态性引物组合,共检测到363个基因位点,其中多态性位点有314个,平均每个引物组合产生18.47个多态性位点,多态性位点百分率为86.50%,显示了较高的多态性比率;各居群遗传相似系数在0.495 9~0.818 2;根据聚类结果可将10个不同居群的明党参分为两大类。结论:明党参不同居群间具有高度的遗传多样性;不同居群的亲缘关系与其地理分布有一定的相关性。
Objective: To study the genetic diversity of Changium smyrnioides and give a reference for utilization of the germplasm.Method: Ten different populations of Ch.smyrnioides were analyzed by the approach of sequence-related amplified polymorphism(SRAP).Genetic similarity coefficient was calculated,and systematic relationships were constructed based on the UPGMA method.Result:Seventeen primer pairs were selected from 160.A total of 363 bands were scored,314 bands of them were polymorphic and the average was 18...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第24期3180-3183,共4页
China Journal of Chinese Materia Medica
基金
国家科技攻关计划项目(2001BA701A592001BA701A62-04)
关键词
明党参
遗传多样性
SRAP
Changium smyrnioides
genetic diversity
SRAP
