摘要
目的:建立超高效液相色谱(UPLC)法测定鸡眼睛(野鸦椿)中鞣花酸类化合物含量的方法。方法:采用AcquityBEHC18(2.1 mm×100 mm,1.7μm)色谱柱;以乙腈-0.1%磷酸为流动相,梯度洗脱;流速0.2 mL.min-1;检测波长245 nm。结果:鸡眼睛药材中5种鞣花酸类化合物在20 min内得到完全分离并进行了含量测定。方法快速、专属,线性关系、精密度和准确度良好。结论:通过研究制定了鸡眼睛(野鸦椿)药材中鞣花酸类化合物的质量控制方法。
Objective: To develop an ultra performance liquid chromatography(UPLC) method for simultaneous determination of five ellagic acids in Euscaphis japonica.Method: Analysis was carried out on an Acquity BEH C18(2.1 mm×100 mm,1.7 μm) column at 40 ℃ eluted with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phases in gradient elution.The flow rate was 0.2 mL·min-1,and the detection wavelength set was monitored at 245 nm.Result: All calibration curves showed good linear relationship within test rang...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第24期3235-3238,共4页
China Journal of Chinese Materia Medica
基金
贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2007]5014号)
