摘要
目的:探讨大黄总蒽醌含药血清对体外培养LoVo细胞的水通道蛋白基因(AQP4)表达的调节效应。方法:16只SD大鼠随机分为正常组,大黄总蒽醌低、中、高剂量组(1.4,2.5,4.5 g.kg-1.d-1,灌胃),7 d后麻醉处死取血制备含药血清。体外培养LoVo细胞并给予不同剂量大黄总蒽醌含药血清培养液24 h,采用W estern b lot及半定量RT-PCR检测AQP4蛋白及mRNA的表达。结果:结果显示,4.5 g.kg-1.d-1大黄总蒽醌含药血清培养液可抑制LoVo细胞的AQP4蛋白及mRNA的表达(P<0.01)。结论:大黄总蒽醌含药血清可抑制LoVo细胞AQP4基因转录与翻译,提示大黄的泻下作用可能与调节AQP4表达有关。
Objective:To investigate the regulating effects of serum containing total anthraquinone in rheum on the expression of AQP4 in cultured LoVo cells in vitro.Method: Sixteen SD rats were randomly divided into 4 groups respectively treated with 0,1.4,2.5,4.5 g·kg-1·d-1 of total anthraquinone in rheum by intragastric administration.After 7 days of treatment,the rats were anesthetized and celiotomized to prepare the serum containing total anthraquinone in rheum.LoVo cells cultured in vitro were treated with diffe...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第24期3247-3250,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30572385)