6种寒性中药对大鼠肝脏能量代谢的影响

Study of hepatic energy metabolism on rats by six cold property traditional Chinese medicines

目的:探讨6种寒性中药对大鼠肝脏能量代谢影响的共同规律。方法:6种寒性中药(苦参、栀子、黄柏、黄芩、黄连和龙胆)的水提取物分别6.0,7.0,8.4,6.0,7.0,4.0 g.kg-1灌胃大鼠30 d,测定大鼠肝脏Na+-K+-ATP酶、Ca2+-ATP酶,琥珀酸脱氢酶(SDH)的活性,肝糖原含量和肝脏解偶联蛋白2(UCP2)的mRNA表达水平。结果:6种寒性中药能显著降低Na+-K+-ATP酶、Ca2+-ATP酶的活性;6种寒性中药均有使SDH活性降低的趋势,其中黄柏、黄芩、黄连和龙胆的降低有统计学意义;6种寒性中药有升高大鼠肝糖原含量的趋势,但是未达到显著水平;6种寒性中药有降低肝脏UCP2 mRNA水平的趋势,其中以苦参、黄柏、黄芩的降低有显著意义。结论:6种寒性中药可能通过降低肝脏线粒体SDH的活性从而减少ATP的生成,降低肝脏Na+-K+-ATP酶、Ca2+-ATP酶的活性从而减少ATP的消耗,减少肝脏UCP2 mRNA的表达从而减少产热,起到调节肝脏能量代谢的作用。

Objective: To investigate the common rule hepatic energy metabolism on rats by six cold property of traditional Chinese medicines(TCM).Method: The activities of Na+-K+-ATPase,Ca2+-ATPase and SDH,the content of hepatic glycogen,and the mRNA expression of hepatic uncoupling protein2(UCP2)were measured after the rats and been administrated with water extracts of Radix Sophorae Flavescentis,Fructus Gardeniae,Cortex Phellodendri,Radix Scutellariae,Rhizoma Coptidis,Radix Gentianae respectively at 6.0,7.0,8.4,6.0,...