摘要
A cDNA library was constructed using poly (A)+ RNA isolated from -1—15 DPA fibers of upland cotton (Gossypium hirsutum). The cDNA encoding a b-tubulin isoform (designated as GhTub1) was identified through EST search. Northern blot analysis using 3′-UTR of the cDNA as a gene-specific probe was performed to investigate the expression levels of GhTub1 in various organs and in the developing fibers. The results showed that GhTub1 gene was specifically ex-pressed in cotton fiber cells. During fiber development, GhTub1 transcripts accumulated highly at the stage of cell rapid elongation with the highest expression appearing at the time when fiber ex-pansion reaches the peak rate. To probe the in vivo function of GhTub1, its cDNA was cloned in the yeast expression vector pREP1 and transformed into the fission yeast Schizosaccharomyces pombe. Overexpression of GhTub1 in yeast cells caused severe changes in the cell morphology. These results suggest that GhTub1 may play a role in the polar elongation of cotton fibers. To our knowledge, this is the first report on the fiber-specific transcript accumulation of a cotton b-tubulin gene.
A cDNA library was constructed using poly (A)+ RNA isolated from -1—15 DPA fibers of upland cotton (Gossypium hirsutum). The cDNA encoding a b-tubulin isoform (designated as GhTub1) was identified through EST search. Northern blot analysis using 3′-UTR of the cDNA as a gene-specific probe was performed to investigate the expression levels of GhTub1 in various organs and in the developing fibers. The results showed that GhTub1 gene was specifically ex-pressed in cotton fiber cells. During fiber development, GhTub1 transcripts accumulated highly at the stage of cell rapid elongation with the highest expression appearing at the time when fiber ex-pansion reaches the peak rate. To probe the in vivo function of GhTub1, its cDNA was cloned in the yeast expression vector pREP1 and transformed into the fission yeast Schizosaccharomyces pombe. Overexpression of GhTub1 in yeast cells caused severe changes in the cell morphology. These results suggest that GhTub1 may play a role in the polar elongation of cotton fibers. To our knowledge, this is the first report on the fiber-specific transcript accumulation of a cotton b-tubulin gene.
基金
supported by the National Natural Science Foundation of China(Grant No.39880014)
National Special Program for Research and Industrialization of Transgenic Plants(Grant No.J99 A 003).
