Imaging theory and resolution improvement of two-photon confocal microscopy 被引量：3

Imaging theory and resolution improvement of two-photon confocal microscopy

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摘要 The nonlinear effect of two-photon excitation on the imaging property of two-photonconfocal microscopy has been analyzed by the two-photon fluorescence intensity transfer functionderived in this paper. The two-photon fluorescence intensity transfer function in a confocal micros-copy is given. Furthermore the three-dimensional point spread function (3D-PSF) and thethree-dimensional optical transfer function (3D-OTF) of two-photon confocal microscopy are de-rived based on the nonlinear effect of two-photon excitation. The imaging property of two-photonconfocal microscopy is discussed in detail based on 3D-OTF. Finally the spatial resolution limit oftwo-photon confocal microscopy is discussed according to the uncertainty principle. The nonlinear effect of two-photon excitation on the imaging property of two-photon confocal microscopy has been analyzed by the two-photon fluorescence intensity transfer function derived in this paper. The two-photon fluorescence intensity transfer function in a confocal microscopy is given. Furthermore the three-dimensional point spread function (3D-PSF) and the three-dimensional optical transfer function (3D-OTF) of two-photon confocal microscopy are derived based on the nonlinear effect of two-photon excitation. The imaging property of two-photon confocal microscopy is discussed in detail based on 3D-OTF. Finally the spatial resolution limit of two-photon confocal microscopy is discussed according to the uncertainty principle.

作者唐志列杨初平裴红津梁瑞生刘颂豪

出处《Science China Mathematics》 SCIE 2002年第11期1468-1478,共11页 中国科学：数学（英文版）

关键词 two-photon CONFOCAL microscopy resolution. two-photon confocal microscopy resolution

分类号 O431.2 [机械工程—光学工程]

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2002年第11期

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Imaging theory and resolution improvement of two-photon confocal microscopy 被引量：3

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